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mouse anti human igg2  (SouthernBiotech)
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SouthernBiotech mouse anti human igg2
(A) Schematic representation of the anti-commensal antibody assay. In a first step, bacteria targets of a specific strain pool are incubated with 4 serial dilutions of the serum sample allowing serum antibodies to bind bacteria surface antigens. Next fluorescently-labelled, secondary anti-isotype antibodies (anti-IgA, IgG1, <t>IgG2</t> and total Ig) are added allowing the visualization of the bacteria through flow cytometry. (B) An example of the signal measured by flow cytometry for one bacteria pool and four serial dilutions of a serum sample. The first four panels show contour plots of the anti-isotype intensity vs forward scatter parameter. The last panel shows a histogram overlay of the anti-isotype signal intensities. (C) Schematic representation of the Response Index calculation. For every serum sample, bacteria strain pool and isotype the dilution curve of the mean fluorescence intensity (MFI) vs dilution factor are generated on a log-log scale (left panel, grey lines). The mean dilution curve is generated using the average MFI values for all serum samples (left panel, black line). For every sample the difference between its dilution AUC and the mean dilution AUC is calculated (ΔAUC, right panel). The resulting Response Index is calculated as 2ΔAUC. (D) Schematic representation of the NET and TrialNet cohort subjects analyzed in this study.
Mouse Anti Human Igg2, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti human igg2 - by Bioz Stars, 2026-10
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(A) Schematic representation of the anti-commensal antibody assay. In a first step, bacteria targets of a specific strain pool are incubated with 4 serial dilutions of the serum sample allowing serum antibodies to bind bacteria surface antigens. Next fluorescently-labelled, secondary anti-isotype antibodies (anti-IgA, IgG1, IgG2 and total Ig) are added allowing the visualization of the bacteria through flow cytometry. (B) An example of the signal measured by flow cytometry for one bacteria pool and four serial dilutions of a serum sample. The first four panels show contour plots of the anti-isotype intensity vs forward scatter parameter. The last panel shows a histogram overlay of the anti-isotype signal intensities. (C) Schematic representation of the Response Index calculation. For every serum sample, bacteria strain pool and isotype the dilution curve of the mean fluorescence intensity (MFI) vs dilution factor are generated on a log-log scale (left panel, grey lines). The mean dilution curve is generated using the average MFI values for all serum samples (left panel, black line). For every sample the difference between its dilution AUC and the mean dilution AUC is calculated (ΔAUC, right panel). The resulting Response Index is calculated as 2ΔAUC. (D) Schematic representation of the NET and TrialNet cohort subjects analyzed in this study.

Journal: Science immunology

Article Title: Association of HLA-dependent islet autoimmunity with systemic antibody responses to intestinal commensal bacteria in children

doi: 10.1126/sciimmunol.aau8125

Figure Lengend Snippet: (A) Schematic representation of the anti-commensal antibody assay. In a first step, bacteria targets of a specific strain pool are incubated with 4 serial dilutions of the serum sample allowing serum antibodies to bind bacteria surface antigens. Next fluorescently-labelled, secondary anti-isotype antibodies (anti-IgA, IgG1, IgG2 and total Ig) are added allowing the visualization of the bacteria through flow cytometry. (B) An example of the signal measured by flow cytometry for one bacteria pool and four serial dilutions of a serum sample. The first four panels show contour plots of the anti-isotype intensity vs forward scatter parameter. The last panel shows a histogram overlay of the anti-isotype signal intensities. (C) Schematic representation of the Response Index calculation. For every serum sample, bacteria strain pool and isotype the dilution curve of the mean fluorescence intensity (MFI) vs dilution factor are generated on a log-log scale (left panel, grey lines). The mean dilution curve is generated using the average MFI values for all serum samples (left panel, black line). For every sample the difference between its dilution AUC and the mean dilution AUC is calculated (ΔAUC, right panel). The resulting Response Index is calculated as 2ΔAUC. (D) Schematic representation of the NET and TrialNet cohort subjects analyzed in this study.

Article Snippet: Bacteria were then incubated (30 min at 4 °C) with 50 μL of fluorophore-conjugated anti-isotype antibodies (goat anti-human IgG (DyLight 405 conjugated, Jackson ImmunoResearch Laboratories, Inc.), mouse anti-human IgG1 (phycoertherin conjugated, SouthernBiotech), mouse anti-human IgG2 (AF647 conjugated, SouthernBiotech) or mouse anti-human IgA (FITC conjugated, Jackson ImmunoResearch Laboratories, Inc.) ( ).

Techniques: Bacteria, Incubation, Flow Cytometry, Fluorescence, Generated

(A) Box-and-whiskers plot of anti-MET-2 IgG2 response indices (Y axis), segregated by HLA haplotype (X axis). (B) Box-and-whiskers plots of anti-R.faecis IgG2 response indices (Y axis), segregated by HLA haplotype (X axis). (C) Interaction plot of anti-MET-2 IgG2 response indices. (D) Interaction plot of anti-R.faecis IgG2 response indices. (E) Linear regression of anti-MET-2 IgG2 response indices by age. (F) Linear regression of anti-R.faecis IgG2 response indices by age.

Journal: Science immunology

Article Title: Association of HLA-dependent islet autoimmunity with systemic antibody responses to intestinal commensal bacteria in children

doi: 10.1126/sciimmunol.aau8125

Figure Lengend Snippet: (A) Box-and-whiskers plot of anti-MET-2 IgG2 response indices (Y axis), segregated by HLA haplotype (X axis). (B) Box-and-whiskers plots of anti-R.faecis IgG2 response indices (Y axis), segregated by HLA haplotype (X axis). (C) Interaction plot of anti-MET-2 IgG2 response indices. (D) Interaction plot of anti-R.faecis IgG2 response indices. (E) Linear regression of anti-MET-2 IgG2 response indices by age. (F) Linear regression of anti-R.faecis IgG2 response indices by age.

Article Snippet: Bacteria were then incubated (30 min at 4 °C) with 50 μL of fluorophore-conjugated anti-isotype antibodies (goat anti-human IgG (DyLight 405 conjugated, Jackson ImmunoResearch Laboratories, Inc.), mouse anti-human IgG1 (phycoertherin conjugated, SouthernBiotech), mouse anti-human IgG2 (AF647 conjugated, SouthernBiotech) or mouse anti-human IgA (FITC conjugated, Jackson ImmunoResearch Laboratories, Inc.) ( ).

Techniques:

Correlations between isotype-specific ACAb responses (columns, bottom) and islet auto-antibody positive status (rows, left) are shown in heatmap colors. ACAb responses and IABs were each clustered by similarity by hierarchical clustering (dendrograms top and right, respectively). ACAb isotypes: IgA •/ IgG1•/ IgG2 •.

Journal: Science immunology

Article Title: Association of HLA-dependent islet autoimmunity with systemic antibody responses to intestinal commensal bacteria in children

doi: 10.1126/sciimmunol.aau8125

Figure Lengend Snippet: Correlations between isotype-specific ACAb responses (columns, bottom) and islet auto-antibody positive status (rows, left) are shown in heatmap colors. ACAb responses and IABs were each clustered by similarity by hierarchical clustering (dendrograms top and right, respectively). ACAb isotypes: IgA •/ IgG1•/ IgG2 •.

Article Snippet: Bacteria were then incubated (30 min at 4 °C) with 50 μL of fluorophore-conjugated anti-isotype antibodies (goat anti-human IgG (DyLight 405 conjugated, Jackson ImmunoResearch Laboratories, Inc.), mouse anti-human IgG1 (phycoertherin conjugated, SouthernBiotech), mouse anti-human IgG2 (AF647 conjugated, SouthernBiotech) or mouse anti-human IgA (FITC conjugated, Jackson ImmunoResearch Laboratories, Inc.) ( ).

Techniques: